Role of CCRL2 in the pathogenesis of mouse experimental arthritis

Abstract

Event Abstract Back to Event Role of CCRL2 in the pathogenesis of mouse experimental arthritis ANNALISA DEL PRETE1, 2*, Safiye Gonzalvo-Feo2, Li Wang2, Cristina Mazzon2, Eugenio Scanziani3, Annunciata Vecchi2 and Silvano Sozzani1 1 Department of Molecular and Translational Medicine, Italy 2 Istituto Clinico Humanitas IRCCS, Italy 3 University of Milan, Dept of Veterinary Pathology, Hygiene and Public Health,, Italy Rheumatoid Arthritis (RA) is an autoimmune-mediated disease characterized by chronic inflammation and leukocyte recruitment into the inflamed joints. Chemokines and chemokine receptor activation are known to represent a major component of the effector molecules involved in the onset of RA. CCRL2 [Chemokine (CC motif) receptor-like 2], also known as L-CCR, is a seven transmembrane protein that shows a high homology degree with many members of the CC chemokine receptor family. CCRL2 possesses a non-canonical DRYLIVE motif in the second intracellular loop that makes it to resemble like a nonsignaling chemotactic receptor (e.g. D6, DARC, CCX-CKR). CCRL2 is expressed by LPS-stimulated murine macrophages, neutrophils, mast cells, dendritic cells, glial cells, astrocytes and microglia and is up-regulated in human RA synovial neutrophils. In order to elucidate the role of CCRL2 in RA, CCRL2 KO mice were tested in the model of collagen-induced arthritis. Only few CCRL2 KO mice displayed the pathological signs characteristic of arthritis with reduced leukocyte infiltration, synovial hyperplasia and joint erosions and with a significant reduction in visual scoring and paw thickness. Moreover, CCRL2 KO mice showed a marked delay (about ten days) in the onset of the disease and histological features reminiscent of recent joint damage. Conversely, the level of specific anti-collagen II IgG in the serum was similar in CCRL2 KO and control mice. The mechanisms underling the protection of CCRL2 mice in RA models are currently under investigation and will help to define whether CCRL2 may represent a new therapeutic target for RA. Acknowledgements IMI JU-BeTheCure,contract 115142-2 Keywords: CCRL2, atypical chemokine receptors, Rheumatoid arthritis, leukocyte trafficking, Neutrophils Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: DEL PRETE A, Gonzalvo-Feo S, Wang L, Mazzon C, Scanziani E, Vecchi A and Sozzani S (2013). Role of CCRL2 in the pathogenesis of mouse experimental arthritis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00722 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. ANNALISA DEL PRETE, Department of Molecular and Translational Medicine, Brescia, Italy, annalisa.delprete@unibs.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers ANNALISA DEL PRETE Safiye Gonzalvo-Feo Li Wang Cristina Mazzon Eugenio Scanziani Annunciata Vecchi Silvano Sozzani Google ANNALISA DEL PRETE Safiye Gonzalvo-Feo Li Wang Cristina Mazzon Eugenio Scanziani Annunciata Vecchi Silvano Sozzani Google Scholar ANNALISA DEL PRETE Safiye Gonzalvo-Feo Li Wang Cristina Mazzon Eugenio Scanziani Annunciata Vecchi Silvano Sozzani PubMed ANNALISA DEL PRETE Safiye Gonzalvo-Feo Li Wang Cristina Mazzon Eugenio Scanziani Annunciata Vecchi Silvano Sozzani Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.