Role of CB1 cannabinoid receptor in the modulation of pituitary hormone secretion

Abstract

Event Abstract Back to Event Role of CB1 cannabinoid receptor in the modulation of pituitary hormone secretion Ibolya Bodnár1, Márk Oláh1*, Dániel Hechtl1, Tibor Wenger2, Miklós Góth3, Valéria Rettoni4 and György M. Nagy1 1 Neuromorphological and Neuroendocrine Research Laboratory, Hungarian Academy of Sciences–Semmelweis University, Department of Human Morphology and Developmental Biology, Hungary 2 Semmelweis University, Department of Human Morphology and Developmental Biology, Hungary 3 State Health Centre, Division of Internal Medicine, Hungary 4 CONICET, Universidad de Buenos Aires, Facultad de Medicina, Centro de Estudios Farmacológicos y Botánicos, Argentina Background and Aims: It is known that cannabinoids can modulate anterior pituitary hormone secretion through a supposed crosstalk between CB1 receptor (CB1R) and different G-protein coupled receptors (GPCRs). We have investigated the significance of CB1 receptor in dopamine- (DA) and angiotensin II (AII) induced prolactin (PRL) and adrenocorticotroph hormone (ACTH) release as well as ghrelin- and growth hormone-releasing hormone (GHRH) induced growth hormone (GH) release using pituitary cell cultures derived from wild type (WT) and CB1 knock-out (CB1KO) mice. Dispersed anterior lobe cells were used immediately after dispersion in the PRL reverse hemolytic plaque assay (RHPA) and/or following 24 hours culture, then the concentration of PRL, ACTH and GH of the culture media was measured by specific RIA. Cells were treated with DA or TRH and with angiotensin II (AII), AM251 (CB1 antagonist), ghrelin or GHRH in the RHPA and in primary cultures, respectively. Results: DA exhibited similar inhibition on PRL release in both WT and CB1KO animals however the number of PRL cells were almost doubled in CB1KO mice detected in the RHPA. AII could strongly stimulate PRL and ACTH secretion in WT animals, while in the CB1KO group PRL release was absent and ACTH release was enhanced. Pretreatment of cultured cells with AM251 prevented AII induced PRL but not ACTH response in WT mice. Basal release of GH was significantly higher in CB1KO mice but the stimulating effects of ghrelin and GHRH were the same in both groups. Conclusion: Our results clearly show that the lack of CB1R profoundly affects secretagogues induced pituitary hormone release in vitro, therefore, indicates an intra-pituitary role of the endocannabinoid system in hormone release. This work was supported by the OTKA-68170 and 68660, ETT-458/09 and Hungarian–Argentinean Intergovermental S&T Cooperation Programme ARG 20/2006. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Bodnár I, Oláh M, Hechtl D, Wenger T, Góth M, Rettoni V and Nagy GM (2010). Role of CB1 cannabinoid receptor in the modulation of pituitary hormone secretion. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00110 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Apr 2010; Published Online: 23 Apr 2010. * Correspondence: Márk Oláh, Neuromorphological and Neuroendocrine Research Laboratory, Hungarian Academy of Sciences–Semmelweis University, Department of Human Morphology and Developmental Biology, Budapest, Hungary, olahmark@ana2.sote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ibolya Bodnár Márk Oláh Dániel Hechtl Tibor Wenger Miklós Góth Valéria Rettoni György M Nagy Google Ibolya Bodnár Márk Oláh Dániel Hechtl Tibor Wenger Miklós Góth Valéria Rettoni György M Nagy Google Scholar Ibolya Bodnár Márk Oláh Dániel Hechtl Tibor Wenger Miklós Góth Valéria Rettoni György M Nagy PubMed Ibolya Bodnár Márk Oláh Dániel Hechtl Tibor Wenger Miklós Góth Valéria Rettoni György M Nagy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.