Abstract
ObjectiveTo investigate the effect of carbonyl sulfide (COS) on limb ischemia/reperfusion (I/R)-induced acute lung injury (ALI) and the associated mechanism in rats.MethodsALI was induced by bilateral hind limb I/R in Sprague–Dawley (SD) rats. Sixty-four SD rats were randomly divided into the control group, I/R group, I/R + COS group, and I/R + AIR group. We observed the survival rate of the rats and the morphological changes of lung tissues, and we measured the change in the lung coefficient, the expression levels of the intercellular adhesion factor-1 (ICAM-1) protein in lung tissue, the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-lβ, and interleukin (IL)-6 in both lung tissue and serum, and cell apoptosis.ResultsLimb I/R caused significant lung tissue damage. The number of polymorphonuclear neutrophil in alveolar septa, the expression level of the ICAM-1 protein in lung tissue, the expression levels of TNF-α, IL-1, and IL-6 in lung tissue and serum, the lung coefficient, and cell apoptosis all increased. When a low dose of COS gas was administered prior to limb I/R, the variation of the above indicators was significantly reduced, while an increase in the dose of COS did not reduce the lung injury but rather increased the mortality rate.ConclusionCarbonyl sulfide is another new gaseous signaling molecule, and a low dose of exogenous COS may play a protective role in I/R-induced ALI by acting as an anti-inflammatory agent by promoting the production of antioxidants and by inhibiting the expression of adhesion molecule proteins.
Highlights
Limb ischemia is a common clinical pathological sign
We focused our research on sulfurous gases [6,7,8], finding that hydrogen sulfide (H2S) and sulfur dioxide (SO2) have a protective effect on acute lung injury (ALI) induced by I/R in rats through regulation of the production of
Histopathological analyses and lung coefficient The morphological changes in the lung suggested the presence of inflammatory damage after 4 h of limb ischemia and 2 h of reperfusion under the light microscope
Summary
Limb ischemia is a common clinical pathological sign. Restoring blood circulation to the limb is necessary to save the body, but it may aggravate the local tissue ischemia/reperfusion (I/R) injury, cause systemic inflammatory response syndrome when serious, or even cause distant multiple organ dysfunction syndrome. Limb I/R-induced ALI remains a common problem for clinical doctors in the. The discovery of the “gas signaling molecule family” facilitated the emergence of a new era of lung injury research [2]. We focused our research on sulfurous gases [6,7,8], finding that H2S and SO2 have a protective effect on ALI induced by I/R in rats through regulation of the production of
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