Abstract
Hypothesis/aims of study. Myometrial relaxation and contraction require synchronous cellular interactions. At present, it has been established that the coordination of myometrial contractile activity is carried out by a conduction system constructed from gap junctions with intercellular channels. There are no clinical data on inhibiting (nitric oxide synthase) and activating (connexin-43) factors of uterine contractile activity in the myometrium during pregnancy and parturition in the published literature. This study was undertaken to measure the expression levels of nitric oxide synthase, adhesion molecules CD51, CD61, and connexin-43 in the myometrium during pregnancy and parturition; and to assess the role of inhibitory and activating factors in the development of uterine contractile activity.
 Study design, materials and methods. An immunohistochemical study of myometrial biopsy specimens obtained from the lower uterus segment during cesarean section was performed in eight women with a full-term physiological pregnancy, in another eight individuals in the active phase of uncomplicated parturition, and in eight patients with uterine inertia. Integrins (CD51 and CD61 proteins) were used as markers of cell adhesion. Localization and the number of intercellular contacts were assessed by measuring the expression level of connexin-43, with the intensity of oxidative processes assessed by nitric oxide synthase activity.
 Results. In the myometrium, in the active phase of physiological parturition, a three-fold increase in the expression of activating (CD51, CD61, and connexin-43) factors of uterine contractile activity and a five-fold decrease in that of inhibitory (nitric oxide synthase) ones occur compared to those in full-term physiological pregnancy.
 Conclusion. In the pathogenesis of uterine inertia and resistance to labor induction, an important role is played by the decreased expression of adhesion molecules (CD51, CD61) and connexin-43 and the increased expression of nitric oxide synthase in the myometrium.
Highlights
В патогенезе слабости родовой деятельности и резистентности к родостимулирующей терапии важную роль играет снижение содержания в миометрии молекул адгезии CD51, CD61, коннексина-43 и увеличение экспрессии NO-синтазы
It has been established that the coordination of myometrial contractile activity is carried out by a conduction system constructed from gap junctions with intercellular channels
An immunohistochemical study of myometrial biopsy specimens obtained from the lower uterus segment during cesarean section was performed in eight women with a full-term physiological pregnancy, in another eight individuals in the active phase of uncomplicated parturition, and in eight patients with uterine inertia
Summary
ФГБНУ «Научно-исследовательский институт акушерства, гинекологии и репродуктологии им. Клинические работы, посвященные изучению содержания ингибирующих (NO-синтаза) и активирующих (протеин межклеточных контактов коннексин-43) факторов сократительной деятельности матки в миометрии при беременности и в родах, в литературе отсутствуют. Цель — изучить экспрессию NO-синтазы, молекул адгезии CD51, CD61 и протеина межклеточных контактов коннексина-43 в миометрии у женщин при беременности и в родах и на основании этого оценить роль ингибирующих и активирующих факторов в развитии сократительной деятельности матки. Для оценки роли ингибирующих и активирующих факторов сократительной деятельности матки проводили иммуногистохимическое исследование биоптатов миометрия из области нижнего сегмента матки, полученных во время кесарева сечения: у 8 женщин при доношенном сроке физиологической беременности, у 8 рожениц в активную фазу неосложненных родов и у 8 со слабостью родовой деятельности. В миометрии в активную фазу физиологических родов происходит трехкратное повышение экспрессии активирующих (адгезивные молекулы CD51, CD61, белок межклеточных контактов коннексин-43) и пятикратное снижение ингибирующего (NO-синтаза) факторов сократительной деятельности матки по сравнению с таковым при доношенном сроке физиологической беременности. ■ Ключевые слова: слабость родовой деятельности; щелевые контакты; коннексины; NO-синтаза; молекулы адгезии; миометрий
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.