Abstract
The present work describes the bio-based green synthesis and characterization of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Tridax procumbens; the synthesized nanoparticles were used to study their beneficial effect on the growth and metabolism of Vigna radiata. ZnO NPs were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and ultraviolet-visible spectroscopy (UV-Vis spectra). Growth of V. radiata seedlings was measured in terms of shoot length and root length that were treated 20 and 40mg/L concentrations of green synthesized ZnO NPs and constant concentration (50mg/L) of PbCl2. These studies have shown the effect of ZnO NPs in the stimulation of growth as well as physiological and biochemical parameters. Vigna seedlings showed positive effects depending upon the increasing concentrations of ZnO NPs. This study suggests that ZnO NPs can be effectively used to ameliorate the toxicity of Pb in Vigna plants.
Highlights
Nanotechnology is the combination of technology and objects around nanoscales size
The method involves growing of Zinc oxide nanoparticles (ZnO NPs) in a liquid medium containing different reactants, in particular reducing agent present in leaf extract
The signature of bio-molecules as evidenced by Fourier Transform Infrared Spectroscopy (FTIR) spectrum reveals that phytochemicals viz; polyphenols, carboxylic acid, polysaccharide, amino acids and proteins are present on the surface of ZnO NPs
Summary
Nanotechnology is the combination of technology and objects around nanoscales size. It is budding area of science that involves the engineering of tiny particles of different materials. Nanotechnology can be considered as the major tool for upcoming industrial revolution and regarded as the foundation for many biotechnological finding in this twenty first century. The nanoscale materials size range from 1 to 100 nm gives them broader ratio for surface to volume and thereby results in the high reactivity of surface. The higher reactivity and high surface to volume ratio property of NM allows their utility in different field covering material science to biotechnology [1]. Due to the above mentioned uniqueness over bulk materials, NPs were consolidated for various innovative alternatives in numerous industries
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