Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation

Barbara Jana,Jarosław Całka

doi:10.1038/s41598-021-91184-5

Abstract

Uterine inflammation is a very common and serious condition in domestic animals. To development and progression of this pathology often lead disturbances in myometrial contractility. Participation of β1-, β2- and β3-adrenergic receptors (ARs) in noradrenaline (NA)-influenced contractility of the pig inflamed uterus was studied. The gilts of SAL- and E.coli-treated groups were administered saline or E.coli suspension into the uterine horns, respectively. Laparotomy was only done in the CON group. Compared to the period before NA administration, this neurotransmitter reduced the tension, amplitude and frequency in uterine strips of the CON and SAL groups. In the E.coli group, NA decreased the amplitude and frequency, and these parameters were lower than in other groups. In the CON, SAL and E.coli groups, β1- and β3-ARs antagonists in more cases did not significantly change and partly eliminated NA inhibitory effect on amplitude and frequency, as compared to NA action alone. In turn, β2-ARs antagonist completely abolished NA relaxatory effect on these parameters in three groups. Summarizing, NA decreases the contractile amplitude and frequency of pig inflamed uterus via all β-ARs subtypes, however, β2-ARs have the greatest importance. Given this, pharmacological modulation of particular β-ARs subtypes can be used to increase inflamed uterus contractility.

Highlights

Uterine inflammation is a very common and serious condition in domestic animals
In the E. coli-infected porcine uterus, the changes in prostaglandin (PG)F2α, PGE2, PGI2 and leukotriene (LT)C4 synthesis and release as well as the effect of PGI2, LTC4 and LTD4 on uterine contractility depend on the intensity and/or duration of the inflammatory process[32,39,40,41]
The results of the macroscopic and histopathological estimation of the uteri utilized in this study have been previously reported and indicated the development of severe acute endometritis in organs treated with E. coli[33]

Summary

Results

In response to β3-ARs antagonist and NA a significant drop in the amplitude was noted in MYO of the control (NA: 1 0–6, 10–5 M) and inflamed (NA: 10–5 M) uteri (Fig. 4B). Β3-ARs antagonist and NA (10–5 M) significantly increased the amplitude in ENDO/MYO of the control and saline-injected uteri, while it significantly reduced in the inflamed uteri (Fig. 4E). Antagonist and NA (10–5 M) significantly lowered the amplitude in ENDO/MYO of the inflamed uteri compared to other groups (Fig. 4E). After application of these factors, the frequency in ENDO/MYO did not differ significantly between the three groups (Fig. 4F)

Discussion

Conclusion

Materials and methods