Abstract
Complex DNA damage may manifest in double-strand breaks (DSBs) and non-DSB, bistranded, oxidatively induced clustered DNA lesions (OCDLs). Although the carcinogen benzo[α]pyrene (B[α]P) has been shown to induce chromosomal aberrations and transformation of mammary cells, it is not known whether this compound engenders clustered DNA damage. Normal primary breast tissue-derived cells were treated with B[α]P, and the levels of DNA lesions, chromosomal aberrations, total antioxidant capacity (TAC), and reactive oxygen species (ROS) were determined. DNA from cells treated with 2 and 8 µM B[α]P exhibited increases of 3- and 4-fold in APE1 ( p < 0.001), 11- and 19-fold in Endo III ( p < 0.001), and 8- and 15-fold in hOGG1 ( p < 0.001) OCDLs, respectively, compared to the 0 µM B[α]P-treated (control) group. Mammary cells treated with 8 µM B[α]P produced 0.12 aberrations per cell ( p < 0.05) and there was a strong positive correlation ( r = 0.91) between the levels of OCDLs and those of chromosomal aberrations. Finally, TAC was decreased by 25% ( p < 0.02), whereas ROS production increased by 2-fold ( p < 0.02) in cells treated with 8 μM B[α]P compared to the control group. In conclusion, oxidatively induced clustered DNA damage mediated through differential expression of APE1, reduced TAC, and increased ROS may play a significant role in the chemically induced transformation of normal primary mammary cells.
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