Abstract
BackgroundAccumulating studies have focused on the oncogenic and tumor suppressive roles of the newly identified lncRNAs. A novel lncRNA BC040587 in 3q13.31 locus which exists frequent copy number alterations was found to be associated with poor survival of osteosarcoma patients. However, its role in breast cancer (BC) remains unknown. The aim of this study was to examine the expression pattern of BC040587 in BC and to evaluate its biological role and clinical significance in prediction of prognosis.MethodsExpression of BC040587 was analyzed in 20 pairs of BC cancer tissues and adjacent noncancerous tissues (ANCT), also in 151 BC tissues, 9 BC cell lines and one normal breast cell line by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Differences between groups were tested for significance using Student’s t-test (two-tailed). Then we analyzed the potential relationship between BC040587 expression and clinic pathological features of BC patients. The correlation was analyzed by SPSS software.ResultsIt showed that BC040587 expression was down regulated both in BC samples and in BC cell lines compared with corresponding normal control. BC040587 expression was correlated with menopausal status (p = 0.040) and tumor differentiation (p = 0.035). The Kaplan-Meier survival curves indicated that the overall survival (OS) was significantly poor in low BC040587 expression BC patients (p = 0.023). Furthermore, expression of BC040587 was significantly associated with worse prognosis and was shown to be an independent prognostic marker breast cancer (p = 0.032). Our studies indicate that BC040587 may represent a new marker of prognosis in breast cancer.ConclusionOur studies indicate that BC040587 is significantly down-regulated in BC tissues and BC cell lines. BC040587 may represent a new marker of prognosis in breast cancer.
Highlights
Accumulating evidence show that Long non-coding RNAs play more and more important roles in a wide range of biological processes
The p value was 0.01 and much less than 0.05. These data indicated that BC040587 might serve as a tumor suppressor in breast cancer
Compared with normal breast cancer cell line MCF10A, the breast cancer cells had much lower BC040587 expression. These data further suggested that expression of BC040587 was relatively low in breast cancer and BC040587 was a candidate tumor suppressor in breast carcinogenesis
Summary
Accumulating evidence show that Long non-coding RNAs (lncRNAs) play more and more important roles in a wide range of biological processes. Many lncRNAs such as HOTAIR, which has been implicated in several cancers, such as lung cancer [4,20], gastric cancer [21], esophageal squamous cell carcinoma [22], colorectal cancers [23], have already shown their potential as novel independent biomarkers for early diagnosis and prognosis prediction in cancer. LncRNA DD3 is found to be a very sensitive and specific biomarker for the detection of prostate tumor cell [24,25] Another recently reported study have shown that lncRNA HOTTIP and HOXA13, which expression were associated with metastasis and survival in HCC patients revealed their possible role of predictive biomarker of HCC [26]. The aim of this study was to examine the expression pattern of BC040587 in BC and to evaluate its biological role and clinical significance in prediction of prognosis
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