Role of AT1 receptors over the oxidative stress in chronic renal failure (CRF)

Abstract

CRF is a progressive condition marked by deteriorating kidney function over time. Elevated blood pressure, renin‐angiotensin system and the imbalance of antioxidant defenses are involved in the progression of renal diseases. The aim of this study was to evaluate the effects of chronic treatment with angiotensin AT1 receptor blockade (Candesartan) in the oxidative stress and evolution of CRF. Wistar rats were divided in 3 groups: control (C, n=6), CRF (n=6) and CRF + Cand (n=6). CRF was induced by the 5/6 nephrectomy model. Treatment with Cand, 25 mg/Kg VO (2 weeks, daily) started 6 weeks after surgery. Creatinine clearance (CrCl) and TBARS (indicative of oxidative stress) were evaluated. Animals of CRF presented a reduction in CrCl and Cand treatment improves this effect (C 2,1±0,2 x CRF 0,9 ±0,1 x CRF + Cand 1,5±0,2 ml/min). Plasmatic levels of TBARS are raised in CRF and Cand treatment normalized this values (C 2,0±0,2 x CRF 3,6 ±0,06 x CRF +Cand 1,0 ± 0,3 nmoles/ml). These results suggest that blockade of AT1 receptor reduces oxidative stress and improve renal function in CRF rats. The reduction of oxidative stress can be an important factor against progression of renal disease.Supported by: CNPq