Abstract
Purpose: To investigate the role of L-ascorbic acid (LAA) on the survival and its association with nitric oxide (NO) production against hydrogen peroxide-induced oxidative stress in trabecular meshwork (TM) cells. Methods: Primarily cultured human TM cells were exposed to hydrogen peroxide; 10, 100 µM for 2 days, or 1 mM single exposure with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. Flow cytometry using annexin/PI double staining was performed to evaluate apoptosis. Results: Hydrogen peroxide decreased cellular survival significantly in a dose-dependent manner accompanied with decreased NO production. However cellular survival and NO production were increased significantly with co-exposure of LAA. Cellular survival and NO were highly correlated. Flow cytometric analysis revealed that LAA inhibited hydrogen peroxide-induced apoptosis. Conclusions: LAA demonstrated a cytoprotective effect against the hydrogen peroxide-induced oxidative stress accompanied with increased NO production. The cytoprotective effect of LAA may be mediated by preserving NO in TM cells.
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