Abstract
This study was conducted for investigating potentials of methanolic extract (Am. CME) and subsequent fractions of Artemisia macrocephala (A. macrocephala) for the inhibition of acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and cyclo-oxygenase (COX-1 and COX-2). Ellman's assay was followed for the investigation of AChE and BChE inhibitory potentials of Am. CME and subsequent fractions of A. macrocephala while cyclo-oxygenase inhibition was investigated using a COX inhibition assay. For AChE inhibitory assay, chloroform (Am.CHF), ethyl acetate (Am.EtOAc) fractions and Am.CME showed 85.76 ± 1.49, 77.50 ± 0.86 and 71.00 ± 1.15 % inhibition of the enzyme respectively at 1000µg/mL concentration. Similarly, for BChE inhibition assay, Am.CHF, Am.EtOAc and Am. CME showed 82.56±0.68, 73.56 ± 1.56 and 68.55±0.81% inhibition of the enzyme respectively at 1000µg/mL concentration. Am.CHF and Am.CME were found to be most active against COX-1 and COX-2. Against COX-1, AmCHF and Am.CME showed 75.23 ± 0.98 and 73.33 ± 0.46% inhibition respectively at 1000µg/mL concentration. Similarly against COX-2, they showed 81.23 ± 1.23 and 79.40 ± 0.46% respectively at 1000µg/mL concentration. It can be concluded from results that Artemisia macrocephala can be used in the treatment of cognitive disorders, along with neurodegenerative ailments, various neuro-pharmacological ailments and certain painful conditions.
Highlights
Introduction central nervous systemAmongst severalNeurodegenerative disease is a general types of dementia, Alzheimer’s disease terminology used for various diseased (AD) has been common that affect about 20 conditions developing from persistent million people in the world
We have reported its essential oil for AChE and BuChE inhibition assays with remarkable results [23]
All samples showed good to moderate percent enzyme inhibition in a concentration dependent manner
Summary
Among all the tested samples, Am.CHF showed the highest percent acetylcholinesterase inhibition, causing 85.76 ± 1.49 percent inhibition at. 77.50 ± 0.86 and 71.00 ± 1.15 percent inhibition, respectively at 1000 μg/mL concentration. All samples showed good to moderate percent enzyme inhibition in a concentration dependent manner. Am.CHF caused maximum percent butyrylcholinesterase inhibition which was. 0.81 and 67.57 ± 0.82 percent enzyme inhibition respectively at 1000 μg/mL concentration. All samples showed enzyme inhibition in concentration dependent manner and were from moderate to good. Standard galanthamine showed 94.45 ± 2.37 percent inhibition at 1000 μg/mL. Regulating Ach level via AChE and BChE inhibition is considered to be a useful therapeutic approach in treatment of AD and several other types of dementia
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
