Role of AhR and ARNT in recurrent spontaneous abortion

Abstract

Objective To explore the relationship between aryl hydrocar-bon receptor (AhR), aryl hydrocarbon receptor nuclear translocator (ARNT), estradiol (E2), estrogen receptor (ER) and recurrent spontaneous abortion (RSA) through observing the level of serum AhR, ARNT, E2, and AhR, ARNT, ER in decidua and chorionic tissues of the patients with recurrent spontaneous abortion. Methods 64 cases of RSA patients who induced abortion at the Shanxi Dayi hospital from May 2015 to September 2017 were chosen as RSA group, and 30 cases of healthy abortion women of the same period who had born full-term normal fetus were choosen as the normal group. The serum, villi and decidua of each case were collected during abortion. The level of AhR, ARNT, ER of both groups were detected by enzyme-linked immunosorbent assay (ELISA) method. The Serum E2 lever were detected by the method of chemical luminescence. The data of all the patients were analyzed. Results ⑴ The level of ARNT in peripheral blood of RSA group was significantly higher than that of normal group (P 0.05). The levels of AhR and ARNT in villi and decidua were significantly higher in RSA group than in normal group (P 0.05); the ratio of AhR/ER and ARNT/ER in the villi and decidua of the RSA group was higher than that of the normal group (P<0.05). Conclusions Overexpression of AhR and ARNT and low expression of ER in villi and decidua tissues may be related to the occurrence of RSA. Key words: Abortion, habitual/ME; Receptors, aryl hydrocarbon/ME; Aryl hydrocarbon receptor nuclear translocator/ME; Estradiol/ME; Receptors, estrogen/ME