Abstract
: Polar flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be O-glycosylated with a heterogeneous heptasaccharide glycan. Two mutants with altered (light and strong) polar flagella glycosylation still able to produce flagella were previously obtained, as well as mutants lacking the O34-antigen lipopolysaccharide (LPS) but with unaltered polar flagella glycosylation. We compared these mutants, altogether with the wild type strain, in different studies to conclude that polar flagella glycosylation is extremely important for A. hydrophila adhesion to Hep-2 cells and biofilm formation. Furthermore, the polar flagella glycosylation is an important factor for the immune stimulation of IL-8 production via toll receptor 5 (TLR5).
Highlights
The mesophilic Aeromonas hydrophila motility is based on a single polar flagellum expressed constitutively
Our current work suggests that the flagellum is a major adhesin and that motility is an important factor for adhesion
Our results indicate that A. hydrophila polar flagellin glycosylation is important for immure stimulation of IL-8 production via toll receptor 5 (TLR5)
Summary
The mesophilic Aeromonas hydrophila motility is based on a single polar flagellum expressed constitutively. The flagella morphogenesis in A. hydrophila, as in other Gram-negative bacteria, is a complex phenomenon that requires coordinated expression of more than 50 genes encoding structural subunits, regulatory proteins and chemo-sensor machinery. The motility by flagella represents an important advantage in moving towards favorable conditions or in avoiding detrimental. 2014, 15 environments [1], and it allows in this case mesophilic Aeromonas to compete with other microorganisms. Sci. 2014, 15 environments [1], and it allows in this case mesophilic Aeromonas to compete with other microorganisms These bacteria are ubiquitous water-borne bacteria, considered opportunistic pathogens of both aquatic and terrestrial animals, some species being associated with gastrointestinal and extraintestinal human diseases [2]
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