Role of Actinidia Chinensis on suppression of UVA‐mediated oxidative damage In HaCaT cells

Abstract

Advanced glycation end‐products(AGEs) accumulate in human skin serves as the UV photosensitizer, and UVA inducing the formation of photoexcited states of these photosensitizers with subsequent generation of reactive oxygen species (ROS) and more AGEs sensitizers. In this study, we demonstrated that combined action of AGEs and UVA cause higher risk of skin damage and evaluated the potential therapeutic effect of Actinidia Chinensis rind ethyl acetate extract (ACRE‐E) in HaCaT keratinocytes. ACRE‐E exhibited ability to attenuate UVA‐induced ROS generation which was measured by using DCFH‐DA reagent. To quantify AGEs formation, immunostaining of AGEs in UVA irradiated HaCaT cell was performed. Exposure to UVA causing high immunoreactivity in cells but in the presence of ACRE‐E, the immunoreactivity level was lower. This indicated that the ability of ACRE‐E to attenuate ROS generation contribute considerably to the suppression of AGEs formation. Topical application of ACRE‐E may confer protection against UVA‐induce oxidative stress and AGE formationGrant Funding Source: Korea university