Abstract
An economically valuable woody plant species tree bean (Cajanus cajan (L.) Millsp.) is predominantly cultivated in tropical and subtropical areas and is regarded as an important food legume (or pulse) crop that is facing serious sodium ion stress. NAM (N-acetyl-5-methoxytryptamine) has been implicated in abiotic and biotic stress tolerance in plants. However, the role of NAM in sodium ion stress tolerance has not been determined. In this study, the effect of NAM was investigated in the economically valuable woody plant species, challenged with stress at 40 mM sodium ion for 3 days. NAM-treated plants (200 μM) had significantly higher fresh weight, average root length, significantly reduced cell size, increased cell number, and increased cytoskeleton filaments in single cells. The expression pattern of one of 10 Tree bean Dynamic Balance Movement Related Protein (TbDMP), TbDMP was consistent with the sodium ion-stress alleviation by NAM. Using TbDMP as bait, Dynamic Balance Movement Related Kinase Protein (TbDBK) was determined to interact with TbDMP by screening the tree bean root cDNA library in yeast. Biochemical experiments showed that NAM enhanced the interaction between the two proteins which promoted resist sodium ion stress resistance. This study provides evidence of a pathway through which the skeleton participates in NAM signaling.
Highlights
Tree bean is an edible bean crop with the sixth highest production among crops worldwide; it is grown primarily in the tropical and subtropical regions of the world and has a planting area of 7 million hectares and a production volume of 6 million tons (FAOSTAT 2018)
Tree bean is a major grain and legume crop that can withstand Sodium ion stress, and it is widely cultivated in the tropics in saline-alkali areas
Developing an economical and efficient method of promoting Sodium ion stress resistance in tree bean is important for realizing its economic value and requires further exploration
Summary
Tree bean is an edible bean crop with the sixth highest production among crops worldwide; it is grown primarily in the tropical and subtropical regions of the world and has a planting area of 7 million hectares and a production volume of 6 million tons (FAOSTAT 2018). In 2018, the global harvest area of tree bean was approximately 7 million hectares, which was concentrated primarily in Asia (87% of the world harvested area), with India, Myanmar, China, Bangladesh, and Pakistan representing the major producers. Dark red corresponds to the regions with the highest yield (>320 kilotons). The yield of the red area is less than or equal to 320 kilotons, the yield of the orange area is less than 25
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