Role of 14-3-3β protein on ovarian folliculogenesis, steroidogenesis and its correlation in the pathogenesis of PCOS in mice

Abstract

This study was designed to assess for the first time the circulating and ovarian level of 14-3-3β protein in the PCOS mice and the possible correlation between 14-3-3β protein with PCOS related increase in testosterone (HA), insulin levels (HI) and reduced insulin sensitivity in the ovary. PCOS was induced in mice using treatment of letrozole (by oral gavage) for 21 days. Immunohistochemical study showed increased expression of 14-3-3β protein in PCOS ovary compared to the control ovary. The circulating testosterone and insulin levels, together with circulating and ovarian levels of 14-3-3β protein also showed significant increase in PCOS mice compared to the control mice. An increase in 14-3-3β protein was observed positively correlated with circulating testosterone and insulin levels but showed a negative correlation with ovarian expression of insulin receptor protein in PCOS mice. The treatment of 14-3-3β protein in vitro to the normal ovary showed a significant increase in testosterone synthesis but a significant decline in insulin receptor protein expression compared to the vehicle-treated ovary of adult mice. The present study showed the direct role of 14-3-3β protein in increasing testosterone synthesis along with decreasing insulin sensitivity. Thus, 14-3-3β protein may be playing possible role in PCOS pathogenesis.