Robustness of Visualization Methods in Preserving the Continuous and Discrete Latent Structures of High-Dimensional Single-Cell Data

Abstract

Contemporary single-cell technologies produce data with a vast number of variables at a rapid pace, making large volumes of high-dimensional data available. The exploratory analysis of such high dimensional data can be aided by intuitive low dimensional visualizations. In this work, we investigate how both discrete and continuous structures in single cell data can be captured using the recently proposed dimensionality reduction method SONG, and compare the results with commonly used methods UMAP and PHATE. Using simulated and real-world datasets, we observed that SONG preserves a variety of patterns including discrete clusters, continuums, and branching structures. More importantly, SONG produced more/equally insightful visualizations compared to UMAP and PHATE in all considered datasets. We also quantitatively validate the high-dimensional pairwise distance preservation ability of these visualization methods in the low dimensional space for the generated visualizations.