Abstract
Emerging evidences demonstrate that circular RNAs (circRNAs) are abnormally expressed in tumors and could serve as prognostic markers for cancers. However, the expression patterns and clinical implications of circRNAs in non-small cell lung cancer (NSCLC) remain obscure. In this study, we profiled circRNA expressions in 10 pairs of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) after ribosomal RNA-depletion and RNase R digestion to enrich circRNAs. Combining five circRNA computational programs, we found that LUAD and LUSC not only share common expression patterns, but also exhibit distinct circRNA expression signatures. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa_circ_0077837 and hsa_circ_0001821 could serve as potential biomarkers for both LUAD and LUSC, while hsa_circ_0001073 and hsa_circ_0001495 could be diagnostic/subtyping marker for LUAD and LUSC, respectively. Therefore, our findings highlight the important diagnostic potential of circRNAs in NSCLC.
Highlights
85% of lung cancer belong to non-small cell lung cancer (NSCLC), including lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) [1]
Our results showed that LUAD and LUSC share common expression patterns of certain circRNAs, and exhibited distinct circRNA expressions
CircRNA profiling in human lung tumors and their adjacent normal tissues Given that circRNA is of low abundance and high resistance to RNase R, we treated ribosomal RNA-depleted total RNAs with RNase R to degrade linear RNAs and enrich circRNAs (Additional file 1)
Summary
Lung cancer is the leading cause of malignancy-related mortality. Approximately 85% of lung cancer belong to non-small cell lung cancer (NSCLC), including lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) [1]. We employed circRNA sequencing and five circRNA computational programs to identify differentially expressed circRNAs in LUAD and LUSC tissues.
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