RNAi targeting Caenorhabditis elegans α-arrestins has small or no effects on lifespan

Sangsoon Park,Heehwa G Son,Yujin Lee,Dae-Eun Jeong,Seung-Jae V Lee,Yoonji Jung,Wooseon Hwang,Dongyeop Lee,Seon Woo A An,Hae-Eun H Park,Murat Artan

doi:10.12688/f1000research.12337.2

Sangsoon Park, Heehwa G Son + Show 9 more

Open Access

https://doi.org/10.12688/f1000research.12337.2

Copy DOI

Abstract

Background: α-arrestins are a family of proteins that are implicated in multiple biological processes, including metabolism and receptor desensitization. Methods: Here, we sought to examine the roles of α-arrestins in the longevity of Caenorhabditis elegans through an RNA interference screen. Results: We found that knocking down each of 24 out of total 29 C. elegans α-arrestins had small or no effects on lifespan. Thus, individual C. elegans α-arrestins may have minor effects on longevity. Conclusions: This study will provide useful information for future research on the functional role of α-arrestins in aging and longevity.

Highlights

Introduction5. We have added a new Supplementary Table S1 (Possible RNA interference (RNAi) target prediction), and the previous Supplementary Table S1 is designated as Supplementary Table S2 (Lifespan data of Caenorhabditis elegans α-arrestin RNAi clones)
We showed that RNA interference (RNAi) knockdown of individual C. elegans α-arrestins had small or no effects on lifespan in wild-type or daf-2 mutants
RNAi targeting some α-arrestins might be insufficient for causing strong lifespan phenotypes

Summary

Introduction

5. We have added a new Supplementary Table S1 (Possible RNAi target prediction), and the previous Supplementary Table S1 is designated as Supplementary Table S2 (Lifespan data of Caenorhabditis elegans α-arrestin RNAi clones). We aimed to determine whether any α-arrestins played a role in the lifespan regulation of wild-type or daf-2 mutants. We performed a lifespan assay-based RNAi screen targeting 24 out of 29 C. elegans α-arrestins. We found that knocking down each of the α-arrestins had small or no effects on the lifespan of wild-type and daf-2 mutants. C. elegans α-arrestins may play minor or modulatory roles in lifespan regulation. It will be important to test the roles of α-arrestins in combinatorial manners and/or by using strong loss-of-function mutations in future research

Objectives

Results

Conclusion