Abstract
BackgroundCD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily. CD147 has been implicated in numerous physiological and pathological activities. Enriched on the surface of many tumor cells, CD147 promotes tumor growth, invasion, metastasis and angiogenesis and confers resistance to some chemotherapeutic drugs. In this study, we investigated the possible role of CD147 in the progression of gastric cancer.MethodsShort hairpin RNA (shRNA) expressing vectors targeting CD147 were constructed and transfected into human gastric cancer cells SGC7901 and CD147 expression was monitored by quantitative realtime RT-PCR and Western blot. Cell proliferation, the activities of MMP-2 and MMP-9, the invasive potential and chemosensitivity to cisplatin of SGC7901 cells were determined by MTT, gelatin zymography, Transwell invasion assay and MTT, respectively.ResultsDown-regulation of CD147 by RNAi approach led to decreased cell proliferation, MMP-2 and MMP-9 activities and invasive potential of SGC7901 cells as well as increased chemosensitivity to cisplatin.ConclusionCD147 involves in proliferation, invasion and chemosensitivity of human gastric cancer cell line SGC7901, indicating that CD147 may be a promising therapeutic target for gastric cancer.
Highlights
CD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily
CD147 expression was gradually increased from normal mucosa to carcinomas through hyperplastic or metaplastic mucosa of the stomach, and its expression was positively correlated with tumor size, depth of invasion, lymphatic invasion and expression of ki-67, Matrix metalloproteinases (MMPs)-2, MMP-9 and vascular endothelial growth factor (VEGF) in gastric cancer
CD147 silencing reduces the proliferation of SGC7901 cells we determined the proliferation of SGC7901, SGC7901/Short hairpin RNA (shRNA)-control and SGC7901/shRNA2 respectively
Summary
CD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily. We investigated the possible role of CD147 in the progression of gastric cancer. A better understanding of the molecular mechanisms underlying gastric cancer formation and progression should be helpful in developing more effective treatments for this disease. Zheng et al [12] investigated the role of CD147 in progression and angiogenesis of gastric cancer. The effect of reducing CD147 levels by genetic methods in established gastric cancer cells has not been investigated, the study of which would help understand its role in the malignant phenotype. In this study, we silenced CD147 expression in human gastric cancer cell line SGC7901 by RNA interference (RNAi) to determine its effect on the proliferation and invasion ability as well as the chemosensitivity of SGC7901 cells
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