Abstract
Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.
Highlights
Oilseed rape (Brassica napus L.) is the third most important oilseed crop in the world (Basalma 2008)
A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv
In Arabidopsis, which is in the same family of brassicaceae, several genes including the ALCATRAZ (ALC), INDEHISCENT (IND), SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) and FRUITFUL (FUL) have been shown to be involved in pod dehiscence (Raman et al 2011)
Summary
Oilseed rape (Brassica napus L.) is the third most important oilseed crop in the world (Basalma 2008). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. Keywords BnSHP gene Á Gene silencing Á Oilseed rape Á RNAi Á Pod shattering Introduction
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