Abstract
The effect of the presence of methylated bases in ribosomal RNA on the efficiency with which the RNA forms hybrid complexes with complementary DNA has been investigated. A comparison between methyl-deficient ribosomal RNA, from methionine-deprived and from chloramphenicol-treated Escherichia coli , and mature ribosomal RNA from untreated cells, showed no differences in hybridization efficiency. Evidence for the accumulation of messenger RNA in both methionine-deprived and chloramphenicol-treated cells was obtained.
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