Abstract
RNA trans-splicing joins two or more RNA molecules into one RNA molecule. This process is a variation of the more familiar and common intron removal during nuclear pre-mRNA splicing. Both splicing reactions are a series of cleavage and ligation reactions resulting in the precise excision of introns and in the joining of exons to generate mature RNA. In nuclear pre-mRNA splicing, the introns are flanked by exons on the same RNA molecule; in trans-splicing the exons are on two different RNA molecules. For brevity, we refer to pre-mRNA splicing as cis-splicing.
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