Abstract

RNA sequencing is a molecular technique which utilizes next generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample. This technique is utilized in the detection of changes in gene expression. Potentially malignant oral disorders are one of the most troublesome lesions seen in the oral cavity which predisposes to the development of oral cancer. Though there are many methods employed in the diagnosis of these disorders, biopsy followed by histological examination is the gold standard procedure followed in the diagnosis. RNA sequencing has been receiving attention among researchers. Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma. The article attempts to summarize the progress in RNA sequencing pertaining to Potentially malignant disorders.

Highlights

  • RNA sequencing is a molecular technique which utilizes generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample

  • Malignant oral disorders are one of the most troublesome lesions seen in the oral cavity which predisposes to the development of oral cancer

  • Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma [5, 6]

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Summary

Introduction

RNA sequencing is a molecular technique which utilizes generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample. This technique is utilized in the detection of changes in gene expression [1]. RNA sequencing has greatly replaced cDNA microarray owing to more precise reproduction of using lanes and flow cells. Another added bonus is that this method allows de novo reconstruction of the transcriptome i.e., unknown material can be analyzed [2]. Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma [5, 6]

RNA sequencing
Next generation sequencing
RNA sequencing in potentially malignant oral disorder
RNA sequencing in leukoplakia
RNA sequencing in oral submucous fibrosis
RNA sequencing in oral lichen planus
Scope of RNA sequencing in potentially malignant oral disorders
10. RNA sequencing aiding in generation of molecular signature
10.1 Sensitivity and specificity
10.2 RNA therapeutics
10.3 Monitoring the prognosis and prediction of recurrence
10.4 RNA sequencing-a tool for research continuum
11. Conclusion
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