Abstract
RNA sequencing is a molecular technique which utilizes next generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample. This technique is utilized in the detection of changes in gene expression. Potentially malignant oral disorders are one of the most troublesome lesions seen in the oral cavity which predisposes to the development of oral cancer. Though there are many methods employed in the diagnosis of these disorders, biopsy followed by histological examination is the gold standard procedure followed in the diagnosis. RNA sequencing has been receiving attention among researchers. Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma. The article attempts to summarize the progress in RNA sequencing pertaining to Potentially malignant disorders.
Highlights
RNA sequencing is a molecular technique which utilizes generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample
Malignant oral disorders are one of the most troublesome lesions seen in the oral cavity which predisposes to the development of oral cancer
Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma [5, 6]
Summary
RNA sequencing is a molecular technique which utilizes generation sequencing to identify and quantify ribonucleic acid (RNA) in a given sample. This technique is utilized in the detection of changes in gene expression [1]. RNA sequencing has greatly replaced cDNA microarray owing to more precise reproduction of using lanes and flow cells. Another added bonus is that this method allows de novo reconstruction of the transcriptome i.e., unknown material can be analyzed [2]. Many studies have been conducted to analyze the application of RNA sequencing in the diagnosis of PMODs as well as in the malignant transformation to oral squamous cell carcinoma [5, 6]
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