Abstract
Campylobacter jejuni is a foodborne pathogen and an important contributor to gastroenteritis in humans. C. jejuni readily forms biofilms which may play a role in the transmission of the pathogen from animals to humans. Herein, we present RNA sequencing data investigating differential gene expression in biofilm and planktonic C. jejuni These data provide insight into pathways which may be important to biofilm formation in this organism.
Highlights
Campylobacter jejuni is a foodborne pathogen and an important contributor to gastroenteritis in humans
Planktonic cell samples were prepared by inoculating heart infusion broth with overnight growth (Oxoid, United Kingdom) for a period of 12 hours under microaerobic conditions at 42°C with shaking
Biofilms were prepared by inoculating Mueller-Hinton broth with an overnight growth (Oxoid, United Kingdom) and allowing biofilm to form statically on the surface in glass petri dishes under aerobic conditions at 42°C
Summary
Campylobacter jejuni is a foodborne pathogen and an important contributor to gastroenteritis in humans. The wild-type C. jejuni strain 11168-O, used in this study, was provided by Diane Newell [7]. Strains were initially grown microaerobically overnight at 42°C on Columbia blood agar with Skirrow supplement. Planktonic cell samples were prepared by inoculating heart infusion broth with overnight growth (Oxoid, United Kingdom) for a period of 12 hours under microaerobic conditions at 42°C with shaking.
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