Abstract
In this research, we used RNA sequencing (RNA-seq) to analyze 23 single cell samples and 2 bulk cells sample from human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. The results from the research demonstrated that there were big differences between two cell lines. Adult bone mesenchyme stem cell lines showed a strong trend on the blood vessel differentiation and cell motion, 48/49 vascular related differential expressed genes showed higher expression in adult bone mesenchyme stem cell lines (Abmsc) than fetal bone mesenchyme stem cell lines (Fbmsc). 96/106 cell motion related genes showed the same tendency. Further analysis showed that genes like ANGPT1, VEGFA, FGF2, PDGFB and PDGFRA showed higher expression in Abmsc. This work showed cell heterogeneity between human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. Also the work may give an indication that Abmsc had a better potency than Fbmsc in the future vascular related application.
Highlights
Mesenchymal stem cells (MSCs) are kind of self-renewal cells which are characterized by their multi-potential to differentiate into different cell types [1]
We detected the expression of adult bone mesenchyme stem cell lines (Abmsc), fetal bone mesenchyme stem cell lines (Fbmsc), Abmsc bulk cell and Fbmsc bulk cell with the number of 7434(31.9%), 7126(30.6%), 9611(41.2%) and 9369(40.2%) when compared to 23289 RefSeq genes(Fig 1a)
With the threshold of “P2 or
Summary
Mesenchymal stem cells (MSCs) are kind of self-renewal cells which are characterized by their multi-potential to differentiate into different cell types [1]. The criteria of International Society for Cellular Therapy (ISCT) defined that MSCs can differentiate into skeleton, cartilage, adipose and some other organs in vitro culture [2]. Many researches showed that MSCs can facilitate the angiogenesis by secretion of pro-angiogenesis factors, such as VEGF and HGF. Majority MSCs would die after injected in vivo in the ischemic conditions [5]. With this unique character, many researches applied to MSCs in their survival and pro-angiogenesis capacity in vivo and in vitro research [6]. Some researchers showed that during hypoxia condition, MSC regulated myocardial angiogenesis by increasing the expression of VEGF [7].
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