Abstract
RNA Interference-mediated Silencing of X11α and X11β Attenuates Amyloid β-Protein Levels via Differential Effects on β-Amyloid Precursor Protein Processing
Highlights
The study of amyloidogenic -amyloid precursor protein (APP)1 processing at the gene, protein, and cellular levels has been a major focus of Alzheimer disease neuropathogenesis research since the isolation of the APP gene in 1987
We present the changes in the protein levels of X11␣, X11, APP-FL, APP-C99, and APP-C83 in the cells treated with APBA1 or APBA2 siRNA as a percentage of those in the cells treated with control siRNA
Ilar extent compared with control siRNA treatment: 75 pg/ml (APBA1 siRNA) and 70 pg/ml (DAPT) versus 116 pg/ml. These data indicated that RNA interference (RNAi) silencing of APBA1 affected APP processing and A production in a manner similar to that of DAPT
Summary
The study of amyloidogenic -amyloid precursor protein (APP)1 processing at the gene, protein, and cellular levels has been a major focus of Alzheimer disease neuropathogenesis research since the isolation of the APP gene in 1987 (see reviews in Refs. 1–3). We assessed the effects of RNAi-mediated silencing of APBA1 on APP processing in H4-APP-FL cells by measuring the protein levels of APP-FL, APP-C99, and APP-C83 following APBA1 siRNA treatment.
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