Abstract
Objective To observe interference RNAi silencing of adult rat cardiomyocytes hypoxia- inducible factor- 1 alpha(HIF- 1α)of the experimental possibilities and transfection efficiency. Methods Construction of small interfering RNA(siRNA)targeting HIF- 1αgenes and transfected into cells of adult rats.Reverse transcriptase polymerase chain reaction(RT- PCR)testing HIF- 1αmRNA levels in adult rat cardiac myocytes,methyl thiazol tetrazolium(MTT)method for detection of myocardial cells of rats in different concentrations of cells, transfected with fluorescence microscopy to detect within 24 hours after transfection effect of transfection with control. Results siRNA transfection results showed effectiveness about 73% si - r- HIF- 1αsilence,at which concentration si- r- HIF- 1αand liposome transfection of myocardial cells transfected with best samples. Conclusion HIF- 1αsiRNA can significantly reduce the HIF- 1 alpha expression in adult rat cardiomyocytes,and very high transfection efficiency. Key words: RNA interference; Hypoxia-inducible factor- 1 alpha
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