Abstract
Eclosion hormone (EH) is an important neuropeptide that regulates growth and development. This study predicted the EH gene (HvEH) of Heortia vitessoides Moore (Lepidoptera: Crambidae) from the transcriptome database and its expression patterns were determined using quantitative real-time polymerase chain reaction. HvEH was expressed in all developmental stages and especially in the head area. RNA interference-mediated silencing of HvEH (2 μg/individual) with double-stranded HvEH RNA (dsHvEH) was achieved within 48 hr. Abnormal phenotypes appeared in the pupa and adult stages. dsHvEH injection suppressed pupation and eclosion rates. HvEH expression increased upon treatment with 20-hydroxyecdysone but decreased at extreme temperatures. These results suggest that HvEH plays an essential role in ecdysis and wing formation in H. vitessoides.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
