Abstract
Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the photinus luciferase gene and the renilla luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications with 4'-thioribonucleosides on siRNA against the photinus luciferase were as potent as natural siRNA. On the other hand, modifications with 4'-thioribonucleosides on natural siRNA against the renilla luciferase improved their RNAi activity. These results suggest that 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
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