RNA Deep-Sequencing Analyses for Detection and Characterization of Avian Orthoreovirus and Fowl Adenovirus Co-Infections in Layer Chickens

doi:10.13188/2325-4645.1000046

Abstract

Highlights

As a segmented double-stranded RNA virus, avian orthoreovirus (ARV) is the important species in the Orthoreovirus, one of the 11 genera in the Reoviridae family [1,2,3,4]
Many research studies have indicated that ARV-infections in poultry can cause various clinical symptoms [2,38], severe viral arthritis or tenosynovitis, runting-stunting syndrome, enteric disease and malabsorption syndromes [2,32,33,34]
The newly emerged/emerging ARV field variant strains have been detected in various poultry species including broilers, broiler breeders, layers, turkeys, chukar partridges, guinea fowls, pheasants and quails in PA during the last several years, and severe viral arthritis or tenosynovitis are the most common symptoms seen in ARV-affected poultry [2628,39-41]

Summary

Introduction

As a segmented double-stranded RNA (dsRNA) virus, avian orthoreovirus (ARV) is the important species in the Orthoreovirus, one of the 11 genera in the Reoviridae family [1,2,3,4]. The full genome of ARV is comprised by 10 dsRNA segments which are clustered into three major groups according to mobility in polyacrylamide gel electrophoresis, namely, large segments (L1,L2, and L3), medium segments (M1,M2, and M3), and small segments (S1,S2,S3, and S4) [57]. Each genome segment of ARV is not used directly for viral protein synthesis, but is transcribed to form functional mRNA which is identical to the positive strand of dsRNA [5]. Three of them are major outer capsid proteins (μB,σB and σC) associated with host cell attachment and induction of virus-neutralizing Tang Y1, Lu H2*. ARVs are usually associated with a variety of clinical diseases in poultry but the viral arthritis/tenosynovitis, enteric disease, and immunosuppression have been considered as the primary [14,15,16]

Methods

Results

Conclusion