Abstract
Xenopus laevis histone H4 and H1 genes were transcribed in vitro to generate artificial precursor mRNAs (pre-mRNAs). These pre-mRNAs were microinjected into oocytes, matured oocytes, and unfertilised eggs of Xenopus laevis and their 3′ cleavage and polyadenylation were investigated. In the oocyte nucleus both H4 and H1 pre-mRNAs were 3′ cleaved but were not detectably polyadenylated. In the oocyte cytoplasm there was neither 3′ cleavage nor polyadenylation of these histone pre-mRNAs. When injected into either matured oocytes or unfertilised eggs, the pre-mRNAs underwent 3′ cleavage but this was inefficient when compared to the oocyte nucleus. In addition approximately 50% of the remaining uncleaved pre-mRNA was subject to a polyadenylation activity which added A tails of approximately 70 A residues. In contrast, artificial mouse β-globin pre-mRNAs were not detectably 3′ cleaved or polyadenylated in either microinjected oocytes or unfertilised eggs.
Published Version
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