RLP分画試薬の非結合リポ蛋白成分の新しいHPLCシステムによる検討(第1報)

Abstract

The lipoprotein components of RLP were studied in 54 subjects, whose RLP-cholesterol (C) levels were abnormally high (over 20 mg/dl), using a combination of an improved HPLC method and an immunoabsorption method (immunoaffinity mixed gels coupled with anti human apo A-I and apo B-100 monoclonal antibodies). Normolipidemic and type IIa subjects showed RLP-C levels lower than 20 mg/dl. The RLP-C levels in 18 normolipidemic control subjects were 3.8±1.3 mg/dl (mean±S.D.), indicating that over 98% of the LDL and HDL bound to the immunoafl'inity gels.The serum RLP-C levels and the lipoprotein components in RLP showed almost no differences among the different lots of the immunoaffinity gels.The major components of RLP were shown to be lipoproteins of chylomicron (CM) and VLDL size in type I and V. Lipoproteins of VLDL size were major components in type IIb and IV. RLP in type III was cholesterol-rich and smaller in particle size than that of other types of hyperlipoproteinemia.In hypertriglyceridemia over 250 mg/dl, lipoproteins of CM and VLDL size were shown to be the major components of RLP, which indicated the delayed clearance of larger triglyceride-rich lipoproteins. The RLP-C levels reflect the concentrations of the lipoproteins of CM and VLDL size as an independent marker for lipid testing such as HDL and total cholesterol. Therefore, the determination of serum RLP-C levels and a detailed analysis by HPLC seem to be a useful clinical marker for the detection of abnormal lipid metabolism in hyperlipoproteinemia.