Abstract
Abstract: Recently, the quality of in vitro matured (IVM) oocytes has been improved by numerous studies using animal models. However, the implantation rates of IVM oocytes remain lower than those of in vitro fertilization (IVF). Chromosome abnormalities, which mainly occur during the first meiotic division, are often observed in IVM oocytes. To clarify how the properties of the cumulus cell layers relate to chromosome abnormalities of oocytes during IVM, we investigated the occurrence of aneuploidy and developmental competence using two different media in a mouse model. Maturation rates were significantly lower in denuded oocytes at oocyte collection in both types of maturation media as compared with those of oocytes mechanically denuded just after collection of cumulus-oocyte complex. Fertilization rates and blastocyst rates were lower in Waymouth's MB752/1 medium than in HTF medium. The occurrence of premature chromatid separation (PCS) remarkably increased in Waymouth's MB752/1 medium compared to HTF m...
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