Risk assessment requires several bee species to address species-specific sensitivity to insecticides at field-realistic concentrations

Abstract

In the European registration process, pesticides are currently mainly tested on the honey bee. Since sensitivity data for other bee species are lacking for the majority of xenobiotics, it is unclear if and to which extent this model species can adequately serve as surrogate for all wild bees. Here, we investigated the effects of field-realistic contact exposure to a pyrethroid insecticide, containing lambda-cyhalothrin, on seven bee species (Andrena vaga, Bombus terrestris, Colletes cunicularius, Osmia bicornis, Osmia cornuta, Megachile rotundata, Apis mellifera) with different life history characteristics in a series of laboratory trials over two years. Our results on sensitivity showed significant species-specific responses to the pesticide at a field-realistic application rate (i.e., 7.5 g a.s./ha). Species did not group into distinct classes of high and low mortality. Bumble bee and mason bee survival was the least affected by the insecticide, and M. rotundata survival was the most affected with all individuals dead 48 h after application. Apis mellifera showed medium mortality compared to the other bee species. Most sublethal effects, i.e. behavioral abnormalities, were observed within the first hours after application. In some of the solitary species, for example O. bicornis and A. vaga, a higher percentage of individuals performed some abnormal behavior for longer until the end of the observation period. While individual bee weight explained some of the observed mortality patterns, differences are likely linked to additional ecological, phylogenetic or toxicogenomic parameters as well. Our results support the idea that honey bee data can be substitute for some bee species’ sensitivity and may justify the usage of safety factors. To adequately cover more sensitive species, a larger set of bee species should be considered for risk assessment.