Abstract
We propose an alternative and simple solution to self-referenced digital holographic imaging based on a ring-shaped bifocal lens, without the need of any mirrors, polarizers or spatial light modulators. We discuss the imaging properties of the ring-shaped bifocal lens in self-referenced holography. The easy applicability of this bifocal lens is demonstrated on a realized microscope setup for volumetric observation of freely moving fluorescent objects, based on a conventional light microscope.
Highlights
The aim of our research is to develop a microscope that can detect, localise and image freely moving fluorescent objects within a thick volume in real time
Holographic imaging is based on an interference phenomenon, in which wavefronts are captured to reconstruct the image of the measured objects [4]
Our goal is to prove that the Ring-shaped Bifocal Lens (RBL) is an efficient tool for making holography based fluorescent volume detection, localization and imaging
Summary
The aim of our research is to develop a microscope that can detect, localise and image freely moving fluorescent objects within a thick volume in real time. The realization of such an instrument would result in an immediate industrial benefit, for example, real-time water monitoring systems [1] can be built, where the use of self-referenced holography [2, 3] is undoubtedly very profitable. Such a light source can be a laser [9], a LED [10], an electron gun [11], or some other sources [12] as well
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