Abstract
PCR amplification indicated the minimum infection rate of Rickettsia spp. was 0.66% in Haemaphysalis longicornis ticks collected from Shandong Province, China. Phylogenetic analysis based on the rrs, gltA, ompA, and ompB genes indicated that the ticks carried R. japonica, Candidatus Rickettsia longicornii, and a novel Rickettsia species related to R. canadensis.
Highlights
PCR amplification indicated the minimum infection rate of Rickettsia spp. was 0.66% in Haemaphysalis longicornis ticks collected from Shandong Province, China
Phylogenetic analysis based on the concatenated sequences of rrs, gltA, ompB, and ompA showed that Rickettsia clones (J84, J85, and J217) were clustered in the same clade with, but distinct from, R. canadensis; clone J244 was in the same clade as Candidatus Rickettsia longicornii; the remaining 13 clones were in the same clade as R. japonica
These results indicated that clones J84, J85, and J217 were a novel Rickettsia species; clone 244 was Candidatus Rickettsia longicornii; and other clones were R. japonica (Figure)
Summary
PCR amplification indicated the minimum infection rate of Rickettsia spp. was 0.66% in Haemaphysalis longicornis ticks collected from Shandong Province, China. In all the tick pools, we amplified nucleic acid preparations with rickettsial universal primers targeting rrs, gltA, and ompB (B1–B4). We further amplified Rickettsia clones in the tick pools closely related to R. japonica with primers of ompA, an SFG rickettsia unique gene.
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