Rice (Oryza sativa L.) hydraulic conductivity links to leaf venation architecture under well-watered condition rather than PEG-induced water deficit

Abstract

Higher plant hydraulic conductivity (K plant) is vital for plant growth, especially under PEG-induced water deficit stress (PEG-IWDS). Leaf venation architecture is a key determinant of leaf hydraulic conductivity (K leaf) and K leaf is a major component of K plant across different plant species. However, there is little information about (1) varietal difference in leaf vein development in cereal crops, such as rice plants; (2) the effects of PEG-IWDS on leaf vein development; (3) the coordination between leaf venation architecture and K plant as well as K leaf under PEG-IWDS. In the present study, widely cultivated eight rice cultivars were grown hydroponically under well-watered condition (WWC) and PEG-IWDS, simulated by adding 15 % (w/v) PEG6000. Leaf venation architecture, including total longitudinal leaf vein number, leaf vein numbers per unit width (LVNW), vein thickness and leaf mass per area, as well as K plant and K leaf were measured to address above-mentioned questions. The results showed that leaf venation architecture exhibited significant varietal differences and PEG-IWDS significantly increased LVNW while decreased vein thickness. PEG-IWDS suppressed both K plant and K leaf but the decrease was much higher in K plant than K leaf. There was a significant and positive correlation observed between LVNW and K leaf under both WWC and PEG-IWDS but the correlation between LVNW and K plant was only significant under WWC. K leaf was significantly and positively correlated with K plant under WWC but not under PEG-IWDS. It is concluded that K leaf is a major determinant for K plant under WWC but not under PEG-IWDS; therefore, breeding or selecting rice cultivars with high LVNW can improve shoot water supplement under WWC but not under PEG-IWDS condition.