Abstract
Purpose: To investigate the protective potential of rice bran water extract (RBE) from Khao Dawk Mali 105 on pancreatic abnormalities in high-fat diet (HFD)-induced obese rats.Methods: Male Sprague-Dawley rats were divided into 4 groups: control group, HFD group, and HFD group treated with RBE at 2,205 or 4,410 mg/kg/day. After 4 weeks, body weight, glucose homeostatic parameters, and pancreatic fat accumulation were assessed. mRNA expression levels of sterol regulatory element-binding protein-1c (SREBP-1c), insulin receptor substrate-2 (IRS-2), glucose transporter-2 (GLUT-2) and glucokinase (GK) genes in pancreas were also analyzed.Results: Compared with HFD group, two doses of RBE-treated rats significantly (p < 0.05) reversed HFD-induced obesity, hyperglycemia, impaired glucose tolerance and pancreatic triglyceride accumulation in rats. Histological examination of HFD-induced obese rats revealed fat droplets in acinar cells, but these alterations were ameliorated in RBE-treated rats. RBE treatment showed significantly (p < 0.05) decreased SREBP-1c expression, and also significantly (p < 0.05) increased IRS-2, GLUT-2 and GK expressions in pancreas.Conclusion: RBE consumption may attenuate pancreatic abnormalities by inhibiting fat accumulation, as well as enhancing insulin sensitivity and glucose sensing in the pancreas of HFD-induced obese rats.Keywords: Rice bran, Obesity, Pancreatic steatosis, Insulin signaling, Glucose sensor
Highlights
Obesity, especially abdominal obesity, appears to play an important role in the impairment of glucose and lipid metabolism, such as hyperglycemia, insulin resistance, ectopic fat deposition and dyslipidemia [1,2]
Samples were subjected to quantitative amplification using TaqMan probe and primer sets for rat SREBP-1c (Rn01495769_m1), insulin receptor substrate-2 (IRS-2) (Rn01482270_s1), glucose transporter-2 (GLUT-2) (Rn00563565_m1) and GK (Rn00561265_m1)
In agreement with previous reports, we found that after high-fat diet (HFD) feeding, pancreatic IRS-2, GLUT-2 and GK mRNA levels were decreased, suggesting that HFD might interfere with insulin signaling and glucose-sensing pathways in the pancreas
Summary
Especially abdominal obesity, appears to play an important role in the impairment of glucose and lipid metabolism, such as hyperglycemia, insulin resistance, ectopic fat deposition and dyslipidemia [1,2]. Consumption of high-energy diets with increased saturated and/or trans-fats, an important environmental cause of obesity, are associated with the initiation of these metabolic disturbances [2]. The pancreas is an important organ for the regulation of cellular and whole-body energy homeostasis. Pancreatic abnormalities linked to the development of obesity, insulin resistance and type 2 diabetes (T2D) [3,4]. A previous report has indicated that activation of SREBP-1c is essential for impaired insulin secretion and islet mass associated with the accumulation of triglycerides (TG) [7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
