Abstract
ABSTRACTThe objective of this study is to evaluate of rice bran mineral extract (RBM) increases the expression of anagen-related molecules in human dermal papilla (DOCs). Four treatment groups were established to evaluate the efficacy of RBM, including a negative control, positive control (ascorbic acid), RBM and ortho-silicic acid (Si(OH)4) (OSA) group. Three days after the DPCs were administered the various treatments, western blot analysis showed that type I collagen expression was increased 2.5-fold in the OSA group and 4-fold in the RBM group, and ALP expression was increased 1.5-fold in the OSA and RBM group while the expression of fibronectin was increased ~3-fold in the OSA group and 2.5-fold in the RBM group. Also, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group. Furthermore, the expression of IL-1a was decreased by more than 50% in the OSA and RBM groups compared to the negative control. Analysis of mRNA expression by RT-qPCR showed that type I collagen increased 1.2-fold in the OSA- and RBM-treated DPCs, whereas type IV collagen increased 2.7-fold in the OSA group and 3.5-fold in the RBM group. However, TGF-β2 mRNA decreased about 80% in the OSA and RBM groups, respectively. Immunohistochemical staining of the DPCs for versican protein showed a significant increase in the OSA- and RBM-treated groups compared to the negative control. Thus, RBM have a potential to recover of DPCs activity and decreased inflammatory-related markers. It can be expected that hair loss prevention and hair growth enhancement can be expected when RBM is applied as a cosmetic product.
Highlights
Human hair dermal papilla cells (HHDPCs) are essential for both the development and formation of the hair follicle and constitute a reservoir of cells with the potential to differentiate into diverse cell types, such as muscle cells, adipocytes, fibroblasts and Schwann cells [1]
In order to examine the toxicity of rice bran mineral extract (RBM) and OSA on the Dermal papilla (DP), the DPs were cultured with As-2p, OSA (2.4 μg/ml) and RBM (30 μl/ml) without FBS for 72 h
One group was only added with As-2p, OSA, and RBM but the other group was aged by UV irradiation (312 nm) for 20 mJ/cm2 to further compare the negative control and experimental groups (OSA and RBM) before the injection
Summary
Human hair dermal papilla cells (HHDPCs) are essential for both the development and formation of the hair follicle and constitute a reservoir of cells with the potential to differentiate into diverse cell types, such as muscle cells, adipocytes, fibroblasts and Schwann cells [1]. Many studies with animal models indicate the interest in dermal papilla cells (DPCs) as a crucial cell subpopulation of the hair follicle in the regenerative processes of the skin. Flowers and, leaves, stimulated hair follicle growth in vitro and in a rat model [4]. Extract activated a hair growth marker in DPCs and promoted hair growth in mice [5]. Koparal et al demonstrated that vinegar and water extracts of Delphinium staphisagria promoted angiogenesis in vitro in human keratinocyte cells and human umbilical vein endothelial cells, suggesting the potential to promote hair growth [6]
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