Abstract
A method for the preparation of free and membrane-bound ribosomes from mosquito intestinal cells was developed and applied for the quantification of these two ribosome populations during an entire digestive cycle of female Aedes aegypti. It was found that the number of free ribosomes increases up to 24 hr after food uptake and then stays constant for a further 24 hr. The number of bound ribosomes increases up to 36 hr after food uptake. Then both populations decrease and reach the values of the unfed controls after 96 hr of digestive activity. The quantitative pattern of appearance of the membrane-bound ribosomes could be correlated with the enzymatic activity of a defined class of proteins, the trypsin-like enzyme.
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