Ribosome-amplified metabolism, RAMBO, measured by NMR spectroscopy

Abstract

NMR spectroscopy was used to investigate the phenomenon of Ribosome Amplified MetaBOlism or RAMBO, between pyruvate kinase and ribosomes. Because the concentration of ribosomes increase as the cell grows, ribosome binding interactions may regulate metabolic fluxes by altering the distribution of bound and free enzymes. Pyruvate kinase (PK) catalyzes the last step of glycolysis and represents a major drug target to control bacterial infections. The binding of metabolic enzymes to ribosomes creates protein quinary structures with altered catalytic activities.