Abstract
Heat-denatured 16 s ribosomal RNA from Escherichia coli will combine with the total 30 s ribosomal proteins to form partially active 30 s ribosomes. The reconstitution process results in a change in the structure of the RNA, which can be demonstrated by chromatography on columns of methylated albuminkieselguhr. The change in RNA structure requires 30 s proteins and elevated temperatures.
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