Ribosomal Protein RPL10A Contributes to Early Plant Development and Abscisic Acid-Dependent Responses in Arabidopsis.

Rocío Soledad Ramos,María Lorena Falcone Ferreyra,Claudia Patricia Spampinato,Paula Casati

doi:10.3389/fpls.2020.582353

Rocío Soledad Ramos, María Lorena Falcone Ferreyra + Show 2 more

Open Access

https://doi.org/10.3389/fpls.2020.582353

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Abstract

Plant ribosomal proteins play universal roles in translation, although they are also involved in developmental processes and hormone signaling pathways. Among Arabidopsis RPL10 family members, RPL10A exhibits the highest expression during germination and early development, suggesting that RPL10A is the main contributor to these processes. In this work, we first analyzed RPL10A expression pattern in Arabidopsis thaliana using transgenic RPL10Apro:GUS plants. The gene exhibits a ubiquitous expression pattern throughout the plant, but it is most strongly expressed in undifferentiated tissues. Interestingly, gene expression was also detected in stomatal cells. We then examined protein function during seedling establishment and abscisic acid (ABA) response. Heterozygous rpl10A mutant plants show decreased ABA-sensitivity during seed germination, are impaired in early seedling and root development, and exhibit reduced ABA-inhibition of stomatal aperture under light conditions. Overexpression of RPL10A does not affect the germination and seedling growth, but RPL10A-overexpressing lines are more sensitive to ABA during early plant development and exhibit higher stomatal closure under light condition both with and without ABA treatment than wild type plants. Interestingly, RPL10A expression is induced by ABA. Together, we conclude that RPL10A could act as a positive regulator for ABA-dependent responses in Arabidopsis plants.

Highlights

Plant ribosomal proteins exist as families of two or more members, which are incorporated into the ribosome in certain tissues or under particular situations (Byrne, 2009; Moin et al, 2016a)
RPL10A transcript levels in root, cotyledons, hypocotyl, and shoot apical meristem from 1-day-old seedlings are higher than those of RPL10B and RPL10C (Klepikova et al, 2016; Supplementary Figure S1C). These comparative analyses show that RPL10A is the most abundantly expressed gene of the ribosomal protein L10 (RPL10) family members and suggest that RPL10A could play important roles during seed germination and early seedling development
RPL10A was highly expressed in germinated seeds (Figures 1A,B) and early seedlings (Figure 1C), with strong GUS staining in proliferating tissues such as shoot and root apical meristems (Figures 1C,D)

Summary

Introduction

Plant ribosomal proteins exist as families of two or more members, which are incorporated into the ribosome in certain tissues or under particular situations (Byrne, 2009; Moin et al, 2016a). Gene expression is mainly regulated at a translational level and involves the selective and dynamic recruitment of specific mRNA to polysomes (Basbouss-Serhal et al, 2015). In this way, components of the translational machinery, such as ribosomal proteins, are key players in the regulation of the germination process

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