Abstract
Summary Fourteen Streptomyces species were examined for restriction fragment length polymorphisms (RFLPs) of their ribosomal RNA genes. Thirteen of the Streptomyces species studied had been found previously to be closely related by phenetic numerical classification and DNA-DNA hybridization. Considerable variations in the RFLP patterns between species were evident, although a significant number of common fragments were observed. A method was established to increase the number of fragments per strain on a single agarose gel to enlarge the data base available for analysis. Analysis of the RFLPs revealed relationships similar to those obtained in previous studies. RFLP analysis appears to be an accurate and rapid strain identification tool for Streptomyces , although relationships based on RFLPs may be valid only for very closely related Streptomyces strains or species.
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