Year-end Sale % OFF 
Abstract
Abstract Some conditions of RNA synthesis in vitro in isolated mitochondria of Tetrahymena pyriformis were investigated. Sucrose gradient centrifugation analyses and methylated albumin-coated Kieselguhr column chromatography of the RNA synthesized in vitro demonstrate that RNA ranging from 14 S to 18 S, not transfer RNA, was synthesized. Furthermore, the RNA is hybridizable with mitochondrial DNA, and the hybridization is not inhibited by the addition of RNA isolated from the material sedimenting at 105,000 x g after centrifugation of mitochondrial lysate for 1½ to 2 hours. Although net transfer RNA synthesis does not occur, the transfer RNA fraction was labeled if 3H-ATP was used for incorporation, suggesting that the incorporation is due to the addition of 3H-ATP at the terminus of the transfer RNA molecule.
Highlights
Prior treatment of mitochondria with DNase results in the inhibition of over 80% of the total incorporation, most of this inhibition can be ascribed to the incubation of mitochondria at the reaction temperature (Fig. 1)
An addition of exogenous DNA to the reaction mixture shows no enhancement of the incorporation, which is consistent with
(2) and yeast [4] mitochondrial incorporation systems. We find that this decay does not occur at low concentrations of mitochondrial protein in the reaction mix
Summary
Culture-A strain of T. pyriformis (ST) was used. The stock culture was maintained in culture tubes containing about 5 ml of 2$& proteose-peptone-0.1 ‘% yeast extract medium. Eight 2-liter Erlenmeyer flasks, each containing 300 ml of 2 y0 proteose-peptone-0.1 y0 yeast extract medium were inoculated with aliquots of stock culture and maintained at 28 f 0.5’ for a period of 40 to 46 hours, By this method, about 6-ml packed cells in a state of exponential growth can be obtained.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
