Abstract

In wheat leaf and root crude extracts several RNases can be distinguished by pH optima analysis, and SDS-PAGE and activity staining. One acidic RNase (approximately 20 kD in size) and a group of neutral RNases have been identified by electrophoretic separation. Neutral RNases can be divided into salt-stimu- lated (about 24 and 26 kD) and salt-inhibited (about 27kD). Following low temperature treatment, acidic and neutral salt-inhibited RNases remain unchanged, while the neutral salt-stimulated RNases with higher mobility generally increase with respect to the control.

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