Rhythmic neuronal interactions and synchronization in the rat dorsal column nuclei

Abstract

Single-unit and multiunit activities were recorded from dorsal column nuclei of anesthetized rats in order to study the characteristics of the oscillatory activity expressed by these cells and their neuronal interactions. On the basis of their firing rate characteristics in spontaneous conditions, two types of dorsal column nuclei cell have been identified. Low-frequency cells (74%) were silent or displayed a low firing rate (1.9±0.48 spikes/s), and were identified as thalamic-projecting neurons because they were activated antidromically by medial lemniscus stimulation. High-frequency cells (26%) were characterized by higher discharge rates (27.2±5.1 spikes/s). None of them was antidromically activated by medial lemniscus stimulation. Low-frequency neurons showed a non-rhythmic discharge pattern spontaneously which became rhythmic under sensory stimulation of their receptive fields (48% of cases; 4.8±0.23 Hz). All high-frequency neurons showed a rhythmic discharge pattern at 13.8± 0.68 Hz either spontaneously or during sensory stimulation of their receptive fields. The shift predictor analysis indicated that oscillatory activity is not phase-locked to the stimulus onset in either type of cell, although the stimulus can reset the phase of the rhythmic activity of high-frequency cells. Cross-correlograms between pairs of low-frequency neurons typically revealed synchronized rhythmic activity when the overlapping receptive fields were stimulated. Rhythmic synchronization of high-frequency discharges was rarely observed spontaneously or under sensory stimulation. High-frequency neuronal firing could be correlated with the low-frequency neuronal activity or more often with the multiunit activity during sensory stimulation. Moreover, the presence of oscillatory activity modulated the sensory responses of dorsal column nuclei cells, favoring their responses. These findings indicate that thalamic-projecting and non-projecting neurons in dorsal column nuclei exhibited distinct oscillatory characteristics. However, both types of neuron may be entrained into an oscillatory rhythmic pattern when their overlapping receptive fields are stimulated, suggesting that in those conditions the dorsal column nuclei generate a populational oscillatory output to the somatosensory thalamus which could modulate and amplify the effectiveness of the somatosensory transmission.