Abstract
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these activities they sustain development and growth. Being naturally present in multiple isoforms, invertases are known to be highly differentiated and tissue specific in such a way that every isoform is characteristic of a specific part of the plant. In this work, we report the identification of the invertase RhVI1 that was found to be highly expressed in rose petals. A characterization of this protein revealed that RhVI1 is a glycosylated membrane-anchored protein associated with the cytosolic side of the vacuolar membrane which occurs in vivo in a monomeric form. Purification yields have shown that the levels of expression decreased during the passage of petals from buds to mature and pre-senescent flowers. Moreover, the activity assay indicates RhVI1 to be an acidic vacuolar invertase. The physiological implications of these findings are discussed, suggesting a possible role of this protein during anthesis.
Highlights
In the presence of sunlight and water, plants are able to reduce carbon dioxide into sugars, sustaining in this way their growth and development
The whole membrane fraction from rose petals was isolated and characterized by SDS-PAGE (Fig. 1). These experiments led to a reproducible pattern of bands representative of the proteome associated with the petal’s membranes, and, as expected, showed that, in these fractions, several proteins were more expressed when compared with the expression levels of the residual proteome components (Fig. 1)
These results indicated that membrane solubilization leads to a sample enriched in one of the proteins associated with the membrane fraction
Summary
In the presence of sunlight and water, plants are able to reduce carbon dioxide into sugars, sustaining in this way their growth and development These sugars, in particular sucrose, are partitioned between photosynthetically and non-photosynthetically active tissues (Tang et al, 1999; Gonzalez et al, 2005; Sergeeva et al, 2006; Wang et al, 2010). Invertases are enzymes that are able to cleave the oxygen bridge between two hexose units releasing them in their free form (Sturm, 1999) These enzymes are encoded by different genes and exist in several isoforms (Sturm, 1996; Tymowska-Lalanne and Kreis, 1998) which are known to be tissue-specific (Godt and Roitsch, 1997; Sturm et al, 1999).
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