Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro.

Xiao-Hong Du,Jian-Bo Song,Ru-Ru Sun,Xin Kang,Guo-Hui Liu,Yan Zhi,Qing-Jun Chen,Yan Xie

doi:10.1155/2020/5834502

Abstract

Rhubarb-Aconite Decoction (RAD), a famous Chinese medicine prescription, has been widely used for treating intestinal injury. However, the effect of RAD on intestinal epithelial cells is unclear. The aim of this study was to investigate the effects of RAD drug-containing serum on the oxidative stress injury and inflammatory response induced by endotoxin (ET) in Caco-2 cells in vitro. Lipid peroxide malondialdehyde (MDA), lactate dehydrogenase (LDH), caspase-11, tumor necrosis factor-α(TNF-α), interleukin-3(IL-3), and cytokeratin (CK)18, adenosine triphosphate (ATP) activity, and intracellular free calcium ion levels were measured. The results showed that ET triggered the activation of caspase-11 and the massive release of TNF-α, increased the inhibitory rate of cell growth, MDA, and LDH expressions in Caco-2 cells. Moreover, RAD drug-containing serum could inhibit caspase-11 activation, decrease the release of TNF-α and IL-3, reduce intracellular free calcium ion, and enhance CK 18 expression and ATP activity. These novel findings demonstrated that ET-induced oxidative stress injury and inflammatory response of Caco-2 cells were improved by RAD drug-containing serum, indicating that RAD may be a good choice for the treatment of intestinal injury.

Highlights

Xiao-hong Du,1 Qing-jun Chen,2 Jian-bo Song,3 Yan Xie,4 Yan Zhi,5 Ru-ru Sun,2 Guo-hui Liu,6 and Xin Kang 2
The effect of Rhubarb-Aconite Decoction (RAD) on intestinal epithelial cells is unclear. e aim of this study was to investigate the effects of RAD drugcontaining serum on the oxidative stress injury and inflammatory response induced by endotoxin (ET) in Caco-2 cells in vitro
RAD drug-containing serum could inhibit caspase-11 activation, decrease the release of tumor necrosis factor α (TNF-α) and interleukin 3 (IL-3), reduce intracellular free calcium ion, and enhance CK 18 expression and adenosine triphosphate (ATP) activity. ese novel findings demonstrated that ET-induced oxidative stress injury and inflammatory response of Caco-2 cells were improved by RAD drug-containing serum, indicating that RAD may be a good choice for the treatment of intestinal injury

Summary

Introduction

E aim of this study was to investigate the effects of RAD drugcontaining serum on the oxidative stress injury and inflammatory response induced by endotoxin (ET) in Caco-2 cells in vitro. Ese novel findings demonstrated that ET-induced oxidative stress injury and inflammatory response of Caco-2 cells were improved by RAD drug-containing serum, indicating that RAD may be a good choice for the treatment of intestinal injury. It is necessary to reduce oxidative stress injury and inhibit the inflammatory response to protect the normal structure and function of intestinal epithelial cells. The aim of this study was to investigate the effects of RAD drugcontaining serum on the oxidative stress injury and inflammatory response induced by ET in human Caco-2 cells in vitro

Objectives

Methods

Results

Conclusion